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BMDMs from WT mice were treated with either media alone, lipopolysaccharide, LPS alone, LPS plus nigericin (LPS + NIG), or LPS + NIG plus OLT1177 <t>(NLRP3</t> inhibitor). Nigericin (10 μM) was added to BMDMs after 3.5 hours of stimulation and cytokines were measured in the culture supernatants at 4h. IL-1β (A), CCL3 (B), and MPO (C) were measured in the culture supernatants by the R&D Duo set ELISA kit. Data are presented as mean ± SEM from three independent biological replicates (BMDMs derived from three independent mice). ****P< .0001.
Nlrp3 Inflammasome Formation, supplied by InvivoGen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nlrp3/bio_rxiv__64898__2026__05__31__729061-34-4-13?v=InvivoGen
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nlrp3 inflammasome formation - by Bioz Stars, 2026-07
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BMDMs from WT mice were treated with either media alone, lipopolysaccharide, LPS alone, LPS plus nigericin (LPS + NIG), or LPS + NIG plus OLT1177 <t>(NLRP3</t> inhibitor). Nigericin (10 μM) was added to BMDMs after 3.5 hours of stimulation and cytokines were measured in the culture supernatants at 4h. IL-1β (A), CCL3 (B), and MPO (C) were measured in the culture supernatants by the R&D Duo set ELISA kit. Data are presented as mean ± SEM from three independent biological replicates (BMDMs derived from three independent mice). ****P< .0001.
Nlrp3 Recombinant Rabbit Monoclonal Antibody, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nlrp3 recombinant rabbit monoclonal antibody - by Bioz Stars, 2026-07
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nlrp3  (Huabio Inc)
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BMDMs from WT mice were treated with either media alone, lipopolysaccharide, LPS alone, LPS plus nigericin (LPS + NIG), or LPS + NIG plus OLT1177 <t>(NLRP3</t> inhibitor). Nigericin (10 μM) was added to BMDMs after 3.5 hours of stimulation and cytokines were measured in the culture supernatants at 4h. IL-1β (A), CCL3 (B), and MPO (C) were measured in the culture supernatants by the R&D Duo set ELISA kit. Data are presented as mean ± SEM from three independent biological replicates (BMDMs derived from three independent mice). ****P< .0001.
Nlrp3, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nlrp3/pm42278526-203-30-32?v=Huabio+Inc
Average 86 stars, based on 1 article reviews
nlrp3 - by Bioz Stars, 2026-07
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BMDMs from WT mice were treated with either media alone, lipopolysaccharide, LPS alone, LPS plus nigericin (LPS + NIG), or LPS + NIG plus OLT1177 <t>(NLRP3</t> inhibitor). Nigericin (10 μM) was added to BMDMs after 3.5 hours of stimulation and cytokines were measured in the culture supernatants at 4h. IL-1β (A), CCL3 (B), and MPO (C) were measured in the culture supernatants by the R&D Duo set ELISA kit. Data are presented as mean ± SEM from three independent biological replicates (BMDMs derived from three independent mice). ****P< .0001.
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223 anti nlrp3  (Abmart Inc)
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BMDMs from WT mice were treated with either media alone, lipopolysaccharide, LPS alone, LPS plus nigericin (LPS + NIG), or LPS + NIG plus OLT1177 <t>(NLRP3</t> inhibitor). Nigericin (10 μM) was added to BMDMs after 3.5 hours of stimulation and cytokines were measured in the culture supernatants at 4h. IL-1β (A), CCL3 (B), and MPO (C) were measured in the culture supernatants by the R&D Duo set ELISA kit. Data are presented as mean ± SEM from three independent biological replicates (BMDMs derived from three independent mice). ****P< .0001.
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223 anti nlrp3 - by Bioz Stars, 2026-07
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nlrp3  (Jackson Laboratory)
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BMDMs from WT mice were treated with either media alone, lipopolysaccharide, LPS alone, LPS plus nigericin (LPS + NIG), or LPS + NIG plus OLT1177 <t>(NLRP3</t> inhibitor). Nigericin (10 μM) was added to BMDMs after 3.5 hours of stimulation and cytokines were measured in the culture supernatants at 4h. IL-1β (A), CCL3 (B), and MPO (C) were measured in the culture supernatants by the R&D Duo set ELISA kit. Data are presented as mean ± SEM from three independent biological replicates (BMDMs derived from three independent mice). ****P< .0001.
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primary antibodies against nlrp3  (Affinity Biosciences)
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BMDMs from WT mice were treated with either media alone, lipopolysaccharide, LPS alone, LPS plus nigericin (LPS + NIG), or LPS + NIG plus OLT1177 <t>(NLRP3</t> inhibitor). Nigericin (10 μM) was added to BMDMs after 3.5 hours of stimulation and cytokines were measured in the culture supernatants at 4h. IL-1β (A), CCL3 (B), and MPO (C) were measured in the culture supernatants by the R&D Duo set ELISA kit. Data are presented as mean ± SEM from three independent biological replicates (BMDMs derived from three independent mice). ****P< .0001.
Primary Antibodies Against Nlrp3, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary antibodies against nlrp3 - by Bioz Stars, 2026-07
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anti nlrp3 ag 20b 0014 antibody  (Adipogen)
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BMDMs from WT mice were treated with either media alone, lipopolysaccharide, LPS alone, LPS plus nigericin (LPS + NIG), or LPS + NIG plus OLT1177 <t>(NLRP3</t> inhibitor). Nigericin (10 μM) was added to BMDMs after 3.5 hours of stimulation and cytokines were measured in the culture supernatants at 4h. IL-1β (A), CCL3 (B), and MPO (C) were measured in the culture supernatants by the R&D Duo set ELISA kit. Data are presented as mean ± SEM from three independent biological replicates (BMDMs derived from three independent mice). ****P< .0001.
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nlrp3  (Wanleibio)
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Wanleibio nlrp3
BMDMs from WT mice were treated with either media alone, lipopolysaccharide, LPS alone, LPS plus nigericin (LPS + NIG), or LPS + NIG plus OLT1177 <t>(NLRP3</t> inhibitor). Nigericin (10 μM) was added to BMDMs after 3.5 hours of stimulation and cytokines were measured in the culture supernatants at 4h. IL-1β (A), CCL3 (B), and MPO (C) were measured in the culture supernatants by the R&D Duo set ELISA kit. Data are presented as mean ± SEM from three independent biological replicates (BMDMs derived from three independent mice). ****P< .0001.
Nlrp3, supplied by Wanleibio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nlrp3/pmc13054027-109-12-13?v=Wanleibio
Average 86 stars, based on 1 article reviews
nlrp3 - by Bioz Stars, 2026-07
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BMDMs from WT mice were treated with either media alone, lipopolysaccharide, LPS alone, LPS plus nigericin (LPS + NIG), or LPS + NIG plus OLT1177 (NLRP3 inhibitor). Nigericin (10 μM) was added to BMDMs after 3.5 hours of stimulation and cytokines were measured in the culture supernatants at 4h. IL-1β (A), CCL3 (B), and MPO (C) were measured in the culture supernatants by the R&D Duo set ELISA kit. Data are presented as mean ± SEM from three independent biological replicates (BMDMs derived from three independent mice). ****P< .0001.

Journal: bioRxiv

Article Title: OLT1177 (Dapansutrile) inhibits Gasdermin D-dependent IL-1β Release and Pyroptotic Cell Death in Bone Marrow-derived Macrophages

doi: 10.64898/2026.05.31.729061

Figure Lengend Snippet: BMDMs from WT mice were treated with either media alone, lipopolysaccharide, LPS alone, LPS plus nigericin (LPS + NIG), or LPS + NIG plus OLT1177 (NLRP3 inhibitor). Nigericin (10 μM) was added to BMDMs after 3.5 hours of stimulation and cytokines were measured in the culture supernatants at 4h. IL-1β (A), CCL3 (B), and MPO (C) were measured in the culture supernatants by the R&D Duo set ELISA kit. Data are presented as mean ± SEM from three independent biological replicates (BMDMs derived from three independent mice). ****P< .0001.

Article Snippet: After 3.5 hours, the NLRP3 inflammasome formation was induced with 10 μM nigericin (InvivoGen, San Diego, CA) for 30 minutes.

Techniques: Enzyme-linked Immunosorbent Assay, Derivative Assay

A) BMDMs were treated with lipopolysaccharide, LPS (1μg/mL) and LPS plus nigercin (NIG). Representative western blots show OLT1177 inhibits GSDMD cleavage to GSDMD-N in a dose-dependent manner. (B, C) Densitometric analysis of the GSDMD-N and IL-1β bands from . All concentrations of OLT1177 significantly reduce both GSDMD-N and IL-1β in a dose-dependent manner. D) Western blot shows NLRP3 and β-actin expression in BMDMs. Data are representative of three independent biological replicates (BMDMs derived from three independent mice). ****P< 0.0001.

Journal: bioRxiv

Article Title: OLT1177 (Dapansutrile) inhibits Gasdermin D-dependent IL-1β Release and Pyroptotic Cell Death in Bone Marrow-derived Macrophages

doi: 10.64898/2026.05.31.729061

Figure Lengend Snippet: A) BMDMs were treated with lipopolysaccharide, LPS (1μg/mL) and LPS plus nigercin (NIG). Representative western blots show OLT1177 inhibits GSDMD cleavage to GSDMD-N in a dose-dependent manner. (B, C) Densitometric analysis of the GSDMD-N and IL-1β bands from . All concentrations of OLT1177 significantly reduce both GSDMD-N and IL-1β in a dose-dependent manner. D) Western blot shows NLRP3 and β-actin expression in BMDMs. Data are representative of three independent biological replicates (BMDMs derived from three independent mice). ****P< 0.0001.

Article Snippet: After 3.5 hours, the NLRP3 inflammasome formation was induced with 10 μM nigericin (InvivoGen, San Diego, CA) for 30 minutes.

Techniques: Western Blot, Expressing, Derivative Assay

LPS induces cell activation and expression of the NLRP3 gene. Nigericin is a polyether antibiotic and acts as a potassium ionophore, causing NLRP3 inflammasome activation. Caspase-1 cleaves pro-IL-1β and GSDMD to IL-1β and GSDM-N, respectively. Mature IL-1β then exits the cells through the GSDMD-N pores.

Journal: bioRxiv

Article Title: OLT1177 (Dapansutrile) inhibits Gasdermin D-dependent IL-1β Release and Pyroptotic Cell Death in Bone Marrow-derived Macrophages

doi: 10.64898/2026.05.31.729061

Figure Lengend Snippet: LPS induces cell activation and expression of the NLRP3 gene. Nigericin is a polyether antibiotic and acts as a potassium ionophore, causing NLRP3 inflammasome activation. Caspase-1 cleaves pro-IL-1β and GSDMD to IL-1β and GSDM-N, respectively. Mature IL-1β then exits the cells through the GSDMD-N pores.

Article Snippet: After 3.5 hours, the NLRP3 inflammasome formation was induced with 10 μM nigericin (InvivoGen, San Diego, CA) for 30 minutes.

Techniques: Activation Assay, Expressing